Content determination of seven active components of Eucommiae Cortex in aortic vascular endothelial cells of spontaneously hypertensive rats by UPLC-MS/MS / 中国中药杂志

In the present study, the contents of seven active components [genipinic acid(GA), protocatechuic acid(PCA), neochlorogenic acid(NCA), chlorogenic acid(CA), cryptochlorogenic acid(CCA),(+)-pinoresinol di-O-β-D-glucopyranosid(PDG), and(+)-pinoresinol 4'-O-β-D-glucopyranoside(PG)] of Eucommiae Cortex in aortic vascular endothelial cells of spontaneously hypertensive rats(SHR) were simultaneously determined by ultra-high liquid chromatography-triple quadrupole mass spectrometry(UPLC-MS/MS). The qualified SHR models were selected. The primary aortic endothelial cells(VECs) of rats were separated and cultured by ligation and adherence, followed by subculture. After successful identification, an UPLC-MS/MS method for simultaneously determining the contents of GA, PCA, NCA, CA, CCA, PDG, PG in seven components of Eucommiae Cortex in VECs was established, including specificity, linearity, matrix effect, recovery, accuracy, precision and stability. The established method had the lo-west limit of quantification of 0.97-4.95 μg·L~(-1), accuracy of 87.26%-109.6%, extraction recovery of 89.23%-105.3%, matrix effect of 85.86%-106.2%, and stability of 86.00%-112.5%. Therefore, the established accurate UPLC-MS/MS method could rapidly and simultaneously determine the contents of the seven active components of Eucommiae Cortex in VECs of SHRs, which provided a refe-rence for the study of cellular pharmacokinetics of active components of Eucommiae Cortex extract.

Non-diterpenoid alkaloids in aerial parts of Aconitum carmichaelii / 中草药

Objective: To study the chemical constituents in the aerial parts of Aconitum carmichaelii. Methods: The air-dried arial parts of A. carmichaelii were powdered and extracted with methanol by percolation extraction. After the removal of solvent under reduced pressure, the crude extract was dissolved in 1.5% HCl solution, and then extracted by ethyl acetate to obtain the total crude extract. The compounds were isolated and purified by column chromatography and identified by spectral analyses (MS, 13C-NMR, and 1H-NMR). Results: Fifteen compounds were isolated from A. carmichaelii and characterized as indol-3-carboxylic acid (1), corchoionol C (2), β-sitosterol-3-O-β-D-glucoside-6’-palmitate (3), (+)-pinoresinol (4), (+)-N-formylnorglaucine (5), oxoglaucidaline (6), glaucine (7), (+)-cataline (8), kaempferol-7-O-α-L-rhamnofuranoside (9), kaempferol-3-O-β-(2″-acetyl)-galactopyranoside (10), megastigmane (11), kaempferol-7-O-α-L-arabinoside (12), kaempferol-3-O-β-D-xylopyranoside (13), kaempferol-3-O-β-D- glucopyranoside (14), and quercetin-3-O-β-D-galactopyranoside (15). Conclusion: All compounds are isolated from aerial parts of A. carmichaelii for the first time, and compounds 1-3,5-6,8-15 are isolated from this plant for the first time.

Study on lignans compounds from Lavandula angustifolia / 中草药

Objective: To investigate the lignans compounds constituents of Lavandula angustifolia. Methods: The chemical constituents were isolated and purified by TLC, silica gel, MCI-gel, and RP-HPLC, and their structures were identified by analysis of spectroscopic evidences and physicochemical properties. Results: A total of 11 constituents were isolated from L. angustifolia and elucidated as pinoresinol (1), syringaresinol (2), fraxiresinol-4'-O-β-D-glucopyranoside (3), syringaresinol-4'-O-β-D-glucopyranoside (4), 8-hydroxypinoresinol-4-O-β-D-glucopyranoside (5), rel-(2α,3β)-7-O-methylcedrusin (6), lariciresinol-4'-O-β-D-glucoside (7), (2S,3R)-2,3-dihydro-2-(4-hydroxy-3-methoxyphenyl)-3-hydroxymethyl-7-methoxybenzofuran-5-(trans) propen-1-ol-3-O-β-glucoside (8), (7S,8R)-dihydrodehydrodiconiferyl alcohol-9-β-D-glucopyranoside (9), (7R,8R)-7,8-dihydro-9'-hydroxyl-3'-methoxyl-8- hydroxymethyl-7-(4-hydroxy-3-methoxyphenyl)-1'-benzofuranpropanol-9'-O-β-D-glucopyranoside (10), and (E)-3-((2S,3S)-2-(4- hydroxy-3-methoxyphenyl)-3-hydroxymethyl-7-methoxy-2,3-dihydrobenzofuran-5-yl) allyl-2-hydroxyacetate (11). Conclusion: The 11 compounds are isolated from this plant for first time.

Chemical constituents from Syneilesis aconitifolia (II) / 中草药

Objective: To study the chemical constituents from Syneilesis aconitifolia. Methods: The chemical constituents were isolated by silica gel column chromatography and HPLC, and its structure were identified by their spectral data and physicochemical properties analysis. Results: Eighteen compounds were isolated from methanol extract ethyl acetate extracts of S.aconitifolia with the structures identified as 3β-angeloyoxy-eremophil-6-en-8-oxo-12,15β-diacid (1), quercetin-3-O-α-L-rhamnoside (2), triacontanol (3), 8β-methoxyeremophil-3,7(11)-diene-8α,12(6α,15)-dilactone (4), 3,4-dihydroxybenzoic acid (5), 8-oxo-eremophil-6,9-dien-12-oic acid (6), 8βH-eremophil-3,7(11)-dien-12,8α(14,6α)-diolide (7), 8αH-6α,10β-dihydroxyeremophilenolide (8), pinoresinol (9), 6β,8β,10β-trihydroxyeremophil-7(11)-en-12,8-olide (10), 10α,15-dihydroxy-oplopan-4-one (11), 6α,15α-epoxy-1β,4β-dihydroxyeudesmane (12), caryolane-1,9β-diol (13), (-)-clovane-2,9-diol (14), cis-3-hexenyl-β-D-glucopyranoside (15), kaempferol-3-O-α-L-rhamnoside (16), quercetin-3-O-β-D-glucopyranoside(17) and (-)-oplopan-4-one-10-α-O-β-D-glucoside (18). Conclusion: Compound 1 is a new compound, named as syneilesis acid. Compounds 4-16 and 18are isolated from this plant for the first time.

Chemical constituents from plant of Fritillaria palldiflora / 中草药

Objective: To study the chemical constituents from Fritillaria pallidiflora. Methods: The constituents were isolated from F. pallidiflora and purified by column chromatography, and the structures were identified by spectra analysis and chemical methods. Results: Sixteen compounds were isolated from F. pallidiflora, including phenolic acids, esters, alkaloids, and the structures were identified as 1,4-diphenylbutane (1), cis-cinnamic acid (2), 4-hydroxy-3-methoxybenzaldehyde (3), acetovanillone (4), 9-octadecenoicacidmethylester (5), methyl ferulate (6), 1-O-feruloylglycerol (7), trans-isoferulic acid (8), syringaresinol (9), pinoresinol (10), 2,3-O-diferuloylglycerol (11), 1,3-O-diferuloyl-glycerol (12), cyclo (L-Pro-L-Ala) (13), cyclo (L-Leu-L-Val) (14), bis (diethylene glycol)phthalate (15), and cyclo-(Phe-Val) (16). Conclusion: All compounds are isolated from F. pallidiflora for the first time, and componds 1, 3-8, 10-13, 15, and 16 are isolated from the genus of Fritillaria for the first time.

Quality Standards of Salt Eucommia Dispensing Granules / 医药导报

Objective To establish the quality standards for salt eucommia dispensing granules. Methods The extractives were obtained by alcohol extraction method. HPLC was applied for the determination of pinoresinol diglucoside in dispensing granules. HPLC fingerprints were established by the contrast of Agilent 1260 HPLC, Waters HPLC and various chromatogram column. Results Pinoresinol diglucoside showed a good linear relationship ( Y = 2. 9594X + 3. 2825,R2 =0.9999) at 102.8-2056.0 mg?L-1 with average recovery of 99.85％ (RSD = 0.31％,n = 9). Conclusion The method is easy-operated and accurate,which has a good specificity for the quality control of common salt eucommia dispensing granules.

Study of chemical components from Lianhua Qingwen Capsule (I) / 中草药

Objective To study the chemical constituents of Lianhua Qingwen Capsule (LQC). Methods The compounds were isolated and purified by column chromatography over silica gel and sephadex LH-20, and preparative RP-HPLC. Their structures were elucidated by physicochemical properties and spectral analyses. Results Twenty compounds were isolated and identified as aloe-emodin (1), phillygenol (2), cepharanone B (3), pinoresinol (4), epipinoresinol (5), pinoresinol monomethyl ether (6), piperolactam A (7), (E)-ethyl 3-(4-hydroxyphenyl) acrylate (8), formononetin (9), isoliquiritigenin (10), naringenin (11), kaempferol (12), citreorosein (13), rhein (14), physcion-8-O-β-D-glucopyranoside (15), 4,5-dioxodehydroasimilobine (16), kaempferol-3-O-α- L-ramnopyranosyl-4″-O-E-(4-hydroxy)-cinnamoyl (17), chrysophanol-1-O-β-D-glucopyranoside (18), chrysophanol-8-O-β-D- glucopyranoside (19), and emodin-8- O-β-D-glucopyranoside (20). Conclusion Compounds 2, 3, 5-17, 19, and 20 are isolated from LQC for the first time. This study establishes the foundation for explaining the efficient substance of LQC.

Lignans chemical constituents from Diaphragma Juglandis Fructus and activity of inhibiting HIV / 中草药

Objective To study the lignans chemical constituents from Diaphragma Juglandis Fructus and their activity of inhibiting HIV. Methods The constituents were isolated from Diaphragma Juglandis Fructus and purified by column chromatography, and the structures were identified by spectra analysis and chemical methods.The activity of anti-HIV-1 were detected by LEDGF/p75-IN proteins complex ELISA kit. Results Sixteen compounds were isolated from Diaphragma Juglandis Fructus and the structures were identified as (-)-syringaresinol (1), (+)-pinoresinol (2), (+)-(7R,7’R,7″S,7’’’S,8S,8’S,8″S,8’’’S)-4″,4’’’-dihydroxy-3,3’,3″,3’’’,5,5’- hexamethoxy-7,9’;7’,9-diepoxy-4,8″;4’,8’’’-bisoxy-8,8’-dineolignan-7″,7’’’,9″,9’’’-tetraol (3), 2,3-dihydroxy-1-(4’-hydroxy-3’-methoxy- phenyl)-propan-1-one (4), 3-hydroxy-1-(4’-hydroxy-3’-methoxyphenyl)-propan-1-one (5), 3’,4’-dimethoxyphenylpropanediol (6), (2S)-3,3-di-(4-hydroxy-3-methoxyphenyl)-propane-1,2-diol (7), 3-hydroxy-1-(4’-hydroxy-3’,5’-dimethoxy phenyl)-propan-1-one (8), (1R,5R,6R)-6-{4’-O-[8″-(7″-(4″-hydroxy-3″-methoxyphenyl)) glyceol]-3’,5’-dimethoxyphenyl}-3,7-dioxabicyclo [3.3.0] octan-2-one (9), curcasinlignan B (10), evofolin-B (11), (7S,8R)-dihydrodehydrodiconiferyl alcohol (12), pinnatifidanin C I (13), (+)-(7S,8S)-4,1’-dihydroxy-3,3’,5’-trimethoxy-7,8,9-trinor-8,4’-oxyneolignan-7,9-diol (14), dysosmarol (15), and 1-(4’-hydroxy-3’-methoxyphenyl)-2- [4″-(3-hydroxypropyl)-2″,6″-dimethoxyphenoxy]-propane-1,3-diol (16). Conclusion All compounds are isolated from Diaphragma Juglandis Fructus for the first time. Compound 13 has the potential activity of inhibiting HIV-1.

Chemical constituents from leaves of Magnolia delavayi / 中草药

Objective To study the chemical constituents from the leaves of Magnolia delavayi. Methods The chemical constituents were isolated and purified by column chromatography on silica gel, Sephadex LH-20, MCI, and HPLC. Their structures were identified based on spectroscopic data. Results Fourteen compounds were isolated from 95% ethanol aqueous extract of M. delavayi and the structures were identified as (2E)-3,7,11-trimethyl-2,10-dodecadien-l,6,7-triol (1), (2E,6E)-3,7,11-trimethyl-2,6-dodecadien-l,10,11- triol (2), loliolide (3), pinoresinol (4), syringaresinol (5), medioresinol (6), eudesmin (7), phillygenin (8), coniferaldehyde (9), 3,4,5-trimethoxycinnamyl alcohol (10), indole-3-carboxaldehyde (11), indole-3-ethanol (12), 3,4-dimethoxy-benzoic acid (13), and 3,4-dimethoxyphenol (14). Conclusion All compounds are isolated from this plant for the first time. In addition, the 1H- and 13C-NMR spectroscopic data are reported for the first time in the current study.

Chemical constituents from roots of Stelleropsis tianschanica / 中国中药杂志

The constituents from 95% ethanol extract of the roots of Stelleropsis tianschanica were purified by column chromatography techniques, leading to the isolation of 17 compounds. Their structures were elucidated by spectroscopic dataas 5'-methoxy lariciresinol(1), pinoresinol(2), daphnoretin(3), acutissimalignan B(4),(+)-secoisolariciresinol(5),(+)-epipinoresinol(6), 7-methyi-daphnoretin(7), thero-8S-7-methoxysyringylglycerol(8), 1-O-methyl-guaiacylglycerol(9), 2R-22'-ferulic acid ester-2,3-dihydroxypropyl ester(10), vesiculosin(11), 4β,5βH-guai-9,7(11)-dien-12,8-olide-1α,8α-diol(12),(-)-nortrachelogenin(13), 4α,5βH-guai-9,7(11)-dien-12,8-olide-1α,8α-diol(14), matairesinol(15), lariciresinol(16)and isolariciresinol(17). Among them, compounds 1-13 wereobtained for the first time fromthe genus Stelleropsis. Compounds 3, 7, 10-14 were tested for their activation of orphan nuclear receptor TR3 with the immunofluorescence technology in 50 μmol•L⁻¹. The results showed that compound 10 displayed moderate activity with the activity ratio of 76.38%, and the others were only about 50.0%.

Regulative effect of pinoresinol diglucoside on gene of ESF-1 cells collagen secretion / 国际中医中药杂志

Objective To study the pinoresinol diglucoside (PDG) on gene regulation role of ESF-1 cells in collagen secretion, to reveal PDG repair mechanisms on scalded skin.Methods The cells cultured in vitro were divided into the control group, the estradiol group and the three different PDG doses groups. The concentration of the high, medium and low dose groups were 100, 10, 1μmol/L, and that of estradiol group were 10-3μmol/L. The activity of proliferation was detected by MTT. Then collagen type I (Col I), collagen typeⅢ (ColⅢ), tissue inhibitors of metalloproteinase 1 (TIMP-1), tissue inhibitors of metalloproteinase 2 (TIMP-2) and matrix metalloproteinase 1 (MMP-1) expression levels of mRNA after administration of cells were detected by RT-PCR.Results Compared with the control group, the proliferation of ESF-1 cells (0.559 ± 0.027, 0.552 ± 0.034vs. 0.489 ± 0.027,P<0.05) in the estradiol and medium-dose PDG was significantly higher. The expression level of mRNA of ColⅠ(0.958 ± 0.021, 0.929 ± 0.031, 0.916 ± 0.015vs. 0.844 ± 0.022), ColⅢ (0.783 ± 0.038, 0.918 ± 0.021, 0.855 ± 0.017vs. 0.678 ± 0.024), TIMP-1 (0.939 ± 0.025, 0.889 ± 0.036, 0.853 ±  0.015 vs. 0.780 ± 0.023), TIMP-2 (0.507 ± 0.024, 0.655 ± 0.037, 0.572 ± 0.025vs. 0.405 ± 0.062) in the estradiol, low-, medium-dose PDG groups were significantly higher than those in the control group (P<0.05 or P<0.01). Besides, the MMP-1 (0.343 ± 0.038, 0.407 ± 0.046, 0.435 ± 0.037vs.0.519 ± 0.041) mRNA expression level in the middle and low dose PDG groups significantly decrease (P<0.05 orP<0.01). Conclusions The PDG could enhance the activity of ESF-1 cell proliferation, increase the expression of related collagen and tissue inhibitor of metalloproteinases and inhibit that of matrix metalloproteinases to repair scalded skin.

Phenolic chemical constituents of dendrobium aphyllum / 中草药

Objective To study the chemical constituents from ethanol extracts of the stems of Dendrobium aphyllum. Methods Ten compounds were isolated by silica gel, Sephadex LH-20 column chromatography and reversed-phase semi-HPLC. Results Ten compounds were identified by analyzing their spectral data and comparing with the previously reported literatures as 4,7-dihydroxy-2- methoxy-9,10-dihydroxyphenanthrene (1), 2,4-dihydroxy-7-methoxy-9,10-dihydroxyphenanthrene (2), 4-methoxy-2,5,7,9-tetrahydroxy- 9,10-dihydrophe-nanthrene (3), 5-methoxy-4,7,9-trihydroxy-9,10-dihydrophenanthrene (4), pinoresinol (5), eugenol-β-D-glucoside (6), trans-ferulic acid (7), dodecan-1-ol (8), β-sitosterol (9), and β-daucosterol (10). Conclusion All compounds are isolated from this plant for the first time.

Study on chemical constituents from Carpesium faberi / 中草药

Objective: To investigate the chemical constituents from the whole plant of Carpesium faberi. Methods: Compounds were isolated by various chromatographic techniques, including silica gel, ODS, sephadex LH-20, and semi-preparative HPLC, and their structures were identified by comparison of their experimental spectroscopic data with their reported data. Results: The phytochemistry investigation led to the isolation of 12 compounds, and their structures were elucidated as ent-kaurane-3β,16β,17-triol (1), 3-(hydroxy-acetyl)-1H-indole (2), 8,9,10-trihydroxythymol (3), 8-hydroxy-9,10-diisobutyryloxy-thymol (4), neryl-β-D- glucopyranoside (5), (3S)-linalyl-β-D-glucopyranoside (6), (1R,2S,4S,5R)-2,5-dihydroxy-p-menthane (7), luteolin (8), apigenin-7-O-β-D-glucopyranoside (9), medioresinol (10), pinoresinol (11), and a mixture of silybin and isosilybin (12). Conclusion: All compounds except compound 7 are not only isolated from this plant for the first time, but also from this genus for the first time.

Chemical constituents from plant of Stelleropsis tianschanica / 中草药

Objective: To study the chemical constituents from the arerial parts of Stelleropsis tianschanica. Methods: The constituents were isolated and purified by silica gel chromatography repeatedly, and the structures were identified by spectra analysis and chemical methods. Results: Thirteen compounds were isolated from S. tianschanica and the structures were identified as (+)-pinoresinol (1), (-)-pinoresinol (2), 3'-desmethylarctigenin (3), arctigenin (4), pluviatolide (5), umbelliferone (6), 4-(3,4- dimethoxybenzyl)-3-(4-hydroxy-3-methoxybenzyl)-tetrahydrofuran-2-ol (7), daphnogitin (8), daphnetone (9), blumenol B (10), loliolide (11), 4'-hydroxyacetophenone (12), and 4'-hydroxybenzoic acid (13). Conclusion: Compounds 1-13 were all obtained for the first time from the plant of S. tianschanica and the genus Stelleropsis Pobed.

Study on chemical constituents of Artemisia integrifolia / 中草药

Objective: To study the chemical constituents from the aerial parts of Artemisia integrifolia. Methods: The chemical constituents were separated and purified by column chromatographies and HPLC. Their structures were determined on the basis of spectroscopic analyses (1H-NMR, 13C-NMR, 2D-NMR, and MS). Results: Fifteen compounds were isolated from the methanol extract in the aerial parts of A. integrifolia with the structures identified as α-curcumene (1), β-sitosterol-3-O-β-D-glucoside (2), zingiberone (3), 3-(2-hydroxyphenyl) propanoic acid methyl ester (4), (E)-o-hydroxycinnamic acid (5), eupatorin (6), cirsimaritin (7), artemetine (8), loliolide (9), luteolin-7-O-β-D-glucopyranoside (10), (+)-pinoresinol (11), α-spinasterol (12), reynosin (13), 3α-hydroxy-1(10),4,11(13)-diager-12,6α-olide (14), and scopoletin (15). Conclusion: Compounds 1 and 3 are isolated from the plants of Artemisia Linn for the first time and compounds 4-9, 11, 13, and 14 are isolated from this plant for the first time.

A new tetrahydrofuranoid lignan from Genkwa Flos / 中草药

Objective: To study the secondary metabolites of Genkwa Flos (the buds of Daphne genkwa). Methods: The compounds were separated and purified by silica gel chromatography and thin layer chromatography, and their structures were determined by analyses of the physicochemical properties and spectral data. Results: Three lignans were obtained and identified as lariciresinol- 9-O-pentatriacontanoate (1), pinoresinol (2), 4-hydroxysesamin (3) from the ethanol extract of D. genkwa. Conclusion: Compound 1 is a new tetrahydrofuranoid lignan, and known compound 3 is obtained for the first time from this plant.

Simultaneous Determination of Pinoresinol Di-glucopyranoside and Pinoresinol Glucoside in Rat Plasma by HPLC-tandem MS/MS for Pharmacokinetic Study / 中草药·英文版

Objective Pinoresinol di-glucopyranoside (PDG) is one of the main active lignans of Eucommiae Cortex considered to be a high-quality antihypertensive drug. In this study the pharmacokinetic process of PDG and its primary in vivo metabolite pinoresinol glucoside (PG) in the portal and jugular vein were surveyed and evaluated simultaneously. Methods A sensitive high-performance liquid chromatography coupled with tandem quadruple mass spectrometry (HPLC-MS/MS) method and sample preparation protocol were developed and validated in method of selectivity, sensitivity, precision, stability, and extraction recovery for the simultaneous determination of PDG and its primary metabolite PG in rat plasma. The double intubation technique was used to simultaneously collect blood from common jugular vein and hepatic portal vein after single ig administration of PDG. Results Using this method, the quantification linearity ranges of PDG and PG in rat plasma were both 0.05-100 ng/mL. This method was successfully applied to the evaluation of the absolute oral bioavailability of PDG and determination of the pharmacokinetic properties of PDG and PG after ig administration of single dose in rats. The bioavailability of PDG at common jugular vein was 51.3% compared to that of 91.6% at hepatic portal vein. Conclusion We conclude that liver is the major conversion site of PDG to PG.

Chemical Constituents of Ixora philippinensis Merr.

Chemical investigations of the dichloromethane extracts of Ixora philippinensis Merr., a plant endemic to the Philippines, led to the isolation of syringaresinol (1), pinoresinol (2), isoscopoletin (3), squalene (4), β-sitosterol (5a), and stigmasterol (5b) from the stems; and 4, 5a, 5b, lupeol (6), and lutein (7) from the leaves. The structures of 1 and 3 were elucidated by extensive 1D and 2D NMR spectroscopy, while those of 2 and 4-7 were identified by comparison of their NMR data with literature data.

Chemical constituents of Cycas aenigma.

Chemical investigation of Cycas aenigma, a plant endemic to the Philippines, led to the isolation of a rare neolignan, 2-[2-hydroxy-5-(3-hydroxypropyl)-3-methoxyphenyl]-1-(4-hydroxy-3-methoxyphenyl)propane-1,3-diol (1), pinoresinol (2), and fatty alcohols (3) from the leaflets; and triglycerols (4), and a mixture of β-sitosterol (5a) and stigmasterol (5b) from the petiole and rachis. The structure of 1 was elucidated by extensive 1D and 2D NMR spectroscopy, while those of 2-5b were identified by comparison of their 1H and/or 13C NMR data with literature data.

Comparison on contents of four active constituents between slab and branch barks of Eucommia ulmoides / 中草药

Objective: To determine the contents of aucubin, chlorogenic acid, geniposide, and pinoresinol diglucoside in the slab and branch barks of Eucommia ulmoides. Methods: The separation was performed on a Cosmosil C18 (250 mm ×4.6 mm, 5 μm) column with the gradient elution acetonitrile -0.1% phosphoric acid; The flow rate was 0.8 mL/min; The detection wavelength was 208 nm; The column temperature was at 25 ℃. Results: The average content of aucubin: slab bark > branch bark; The average content of chlorogenic acid: branch bark > slab bark; The average content of geniposide: branch bark > slab bark; The average content of pinoresinol diglucosid: slab bark > branch bark. In different origins, the average contents of the above four constituents are more certain differences. Conclusion: The method is simple, rapid, accurate, and there are significant differences in the contents of aucubin, chlorogenic acid, geniposide, and pinoresinol diglucosid from the different parts of E. ulmoides, which would provide the better technique support for the quality control of E. ulmoides.